The role of architecture in the democratisation of South Africa in disadvantaged communities : a design of a civic centre for Mpumalanga Township.

Thesis (M.Arch.)-University of KwaZulu-Natal, Durban, 2010.In the field of architecture the socio-cultural factors have been deterministic in the formation of place, conditions within them and consequently, social relations. Sociologists, anthropologists and environmentalist have advocated that buildings are essentially social and cultural products - King (1980), Rapoport (1969; 1976; 1977) and Bartuska & Young (1994). Architecture that addresses the human socio-cultural factors has been advocated to make a significant contribution to human life; it fosters a sense of belonging, well being and involvement. South Africa has endured years of colonisation and apartheid ruling, this has also reflected on its built environment. It was planned and designed to communicate and reinforce the dominance of the ruling regime which thus transformed the local populace by incorporating them into their political, economic and social value systems. The political shift of 1994 has however (from apartheid to a democratic ruling state) facilitated a renewed interest in acknowledging peoples differences, their unique characteristics and celebrating the diverse nature of a heterogeneous society. The democratisation of South Africa has brought about a major shift in the social and cultural context of the society which in turn has affected the built environment and architecture. It is in this context that this study explores the nature of the transformation, its ideals and principles so to inform the making of environments that help uplift the populace and to integrate our multicultural society while simultaneously celebrating, facilitating and accommodating the diverse cultures of the groups within it. Thus as professionals involved in the design of the built environment, there is an urgent need to identify and understand the socio-culture of society due to the political shift in South Africa in order to orientate in the right direction towards playing a role in the democritisation of South Africa. Hence the topic: The role of architecture in the democritisation of South Africa

Subtle Longitudinal Alterations in Env Sequence Potentiate Differences in Sensitivity to Broadly Neutralizing Antibodies following Acute HIV-1 Subtype C Infection

Broadly neutralizing antibodies (bNAbs) for HIV-1 prevention or cure strategies must inhibit transmitted/founder and reservoir viruses. Establishing sensitivity of circulating viruses to bNAbs and genetic patterns affecting neutralization variability may guide rational bNAbs selection for clinical development. We analyzed 326 single env genomes from nine individuals followed longitudinally following acute HIV-1 infection, with samples collected at ~1 week after the first detection of plasma viremia; 300 to 1,709 days postinfection but prior to initiating antiretroviral therapy (ART) (median = 724 days); and ~1 year post ART initiation. Sequences were assessed for phylogenetic relatedness, potential N- and O-linked glycosylation, and variable loop lengths (V1 to V5). A total of 43 env amplicons (median = 3 per patient per time point) were cloned into an expression vector and the TZM-bl assay was used to assess the neutralization profiles of 15 bNAbs targeting the CD4 binding site, V1/V2 region, V3 supersite, MPER, gp120/gp41 interface, and fusion peptide. At 1 μg/mL, the neutralization breadths were as follows: VRC07-LS and N6.LS (100%), VRC01 (86%), PGT151 (81%), 10-1074 and PGT121 (80%), and less than 70% for 10E8, 3BNC117, CAP256.VRC26, 4E10, PGDM1400, and N123-VRC34.01. Features associated with low sensitivity to V1/V2 and V3 bNAbs were higher potential glycosylation sites and/or relatively longer V1 and V4 domains, including known "signature" mutations. The study shows significant variability in the breadth and potency of bNAbs against circulating HIV-1 subtype C envelopes. VRC07-LS, N6.LS, VRC01, PGT151, 10-1074, and PGT121 display broad activity against subtype C variants, and major determinants of sensitivity to most bNAbs were within the V1/V4 domains. IMPORTANCE Broadly neutralizing antibodies (bNAbs) have potential clinical utility in HIV-1 prevention and cure strategies. However, bNAbs target diverse epitopes on the HIV-1 envelope and the virus may evolve to evade immune responses. It is therefore important to identify antibodies with broad activity in high prevalence settings, as well as the genetic patterns that may lead to neutralization escape. We investigated 15 bNAbs with diverse biophysical properties that target six epitopes of the HIV-1 Env glycoprotein for their ability to inhibit viruses that initiated infection, viruses circulating in plasma at chronic infection before antiretroviral treatment (ART), or viruses that were archived in the reservoir during ART in subtype C infected individuals in South Africa, a high burden country. We identify the antibodies most likely to be effective for clinical use in this setting and describe mutational patterns associated with neutralization escape from these antibodies